Genomics

Dataset Information

299

Genome-wide binding pattern of PU.1 in normal erythroid progenitors and erythroleukemia cells


ABSTRACT: We find that the myeloid master regulatory transcription factor, PU.1, binds to >16,000 sites in both normal and leukemic erythroid cells. Of these bound sites, ~7,000 lie within 2kb of TSS of a gene, suggesting PU.1 may regulate a large number of genes in erythroid cells. Coupling this data with gene expression analysis, we show PU.1 directly regulates several critical signaling pathways in erythroid cells. Assaying PU.1 occupancy in normal and leukemic erythroid cells

ORGANISM(S): Mus musculus  

SUBMITTER: Arthur I Skoultchi   Sandeep N Wontakal  Sandeep Nagaraj Wontakal 

PROVIDER: E-GEOD-21950 | ArrayExpress | 2011-06-23

SECONDARY ACCESSION(S): GSE21950SRP002542PRJNA129391

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

A large gene network in immature erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1.

Wontakal Sandeep N SN   Guo Xingyi X   Will Britta B   Shi Minyi M   Raha Debasish D   Mahajan Milind C MC   Weissman Sherman S   Snyder Michael M   Steidl Ulrich U   Zheng Deyou D   Skoultchi Arthur I AI  

PLoS genetics 20110609 6


PU.1 is a hematopoietic transcription factor that is required for the development of myeloid and B cells. PU.1 is also expressed in erythroid progenitors, where it blocks erythroid differentiation by binding to and inhibiting the main erythroid promoting factor, GATA-1. However, other mechanisms by which PU.1 affects the fate of erythroid progenitors have not been thoroughly explored. Here, we used ChIP-Seq analysis for PU.1 and gene expression profiling in erythroid cells to show that PU.1 regu  ...[more]

Publication: 1/2

Similar Datasets

2011-06-23 | E-GEOD-21949 | ArrayExpress
2012-02-27 | E-GEOD-35384 | ArrayExpress
2012-02-27 | E-GEOD-35385 | ArrayExpress
2012-02-27 | E-GEOD-35379 | ArrayExpress
| GSE21950 | GEO
2007-11-22 | E-GEOD-5654 | ArrayExpress
2006-10-15 | GSE5654 | GEO
2013-02-05 | E-GEOD-44054 | ArrayExpress
| GSE77651 | GEO
2014-04-24 | E-GEOD-37315 | ArrayExpress