Genomics

Dataset Information

10

Discrete Roles of STAT4 and STAT6 Transcription Factors in Tuning Epigenetic Modifications and Transcription during Helper T Cell Differentiation (ChIP-Seq)


ABSTRACT: Signal transducer and activator of transcription 4 (STAT4) and STAT6 are key factors in the specification of helper T cells; however, their direct roles in driving differentiation are not well understood. Using chromatin immunoprecipitation and massive parallel sequencing, we quantitated the full complement of STAT-bound genes, concurrently assessing global STAT-dependent epigenetic modifications and gene transcription using cells from cognate STAT-deficient mice. STAT4 and STAT6 each bound over 4000 genes with distinct binding motifs. Both played critical roles in maintaining chromatin configuration and transcription of a core subset of genes through the combination of different epigenetic patterns. Globally, STAT4 had a more dominant role in promoting active epigenetic marks, whereas STAT6 had a more prominent role in antagonizing repressive marks. Clusters of genes negatively regulated by STATs were also identified, highlighting previously unappreciated repressive roles. Therefore, STAT4 and STAT6 play wide regulatory roles in T helper specification. The roles of STAT proteins to shape T helper cell phenotype was investigated by comparing DNA binding profiles of STAT4 and STAT6 in Th1 and Th2 conditions. The functional outcome of STAT bindings was further evaluated by profiling histone epigenetic marks and gene expression changes between WT and STAT-deficient T cells in Th1 and Th2 conditions.

ORGANISM(S): Mus musculus  

SUBMITTER: Chongzhi Zang   Weiqun Peng  Jonathan Liang  Hong-Wei Sun  Haydee L Ramos  Gustavo Gutierrez-Cruz  Wendy T Watford  John J O’Shea  Lai Wei  Yuka Kanno  Hiroaki Takatori  Hayato Takahashi  Golnaz Vahedi 

PROVIDER: E-GEOD-22104 | ArrayExpress | 2010-06-18

SECONDARY ACCESSION(S): GSE22104SRP002570PRJNA129179

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

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E-GEOD-22104.idf.txt Idf
E-GEOD-22104.processed.1.zip Processed
E-GEOD-22104.processed.2.zip Processed
E-GEOD-22104.processed.3.zip Processed
E-GEOD-22104.processed.4.zip Processed
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Publications

Discrete roles of STAT4 and STAT6 transcription factors in tuning epigenetic modifications and transcription during T helper cell differentiation.

Wei Lai L   Vahedi Golnaz G   Sun Hong-Wei HW   Watford Wendy T WT   Takatori Hiroaki H   Ramos Haydee L HL   Takahashi Hayato H   Liang Jonathan J   Gutierrez-Cruz Gustavo G   Zang Chongzhi C   Peng Weiqun W   O'Shea John J JJ   Kanno Yuka Y  

Immunity 20100601 6


Signal transducer and activator of transcription 4 (STAT4) and STAT6 are key factors in the specification of helper T cells; however, their direct roles in driving differentiation are not well understood. Using chromatin immunoprecipitation and massive parallel sequencing, we quantitated the full complement of STAT-bound genes, concurrently assessing global STAT-dependent epigenetic modifications and gene transcription by using cells from cognate STAT-deficient mice. STAT4 and STAT6 each bound o  ...[more]

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