Dataset Information

Expression profiles of the hippocampus of Rsk2-KO mice

ABSTRACT: Coffin–Lowry Syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes Rsk2, a serine/threonine kinase involved in spatial memory. We analyzed hippocampal gene expression profiles in Rsk2-KO mice to identify changes in molecular pathways. Total RNA was extracted from hippocampi from 6 KO and 6 WT (littermates) 2-month-old male mice. For each genotype, equivalent amounts of RNA from 2 mice were pooled and processed for hybridization to the genome wide oligonucleotide microarray (Murine 430A 2.0 Affymetrix, 22.000 probe sets). Thus, 3 independent pooled samples were hybridized for each genotype. We compared hippocampal gene expression profiles from rsk2-KO and normal littermate mice to identify changes in molecular pathways

ORGANISM(S): Mus musculus

SUBMITTER: André Hanauer Nathalie Rouach Tahir Mehmood Mohamed Ammar Patricia Marques-Pereira Jérémie Sibillec Anne Schneider Solange Pannetier Doulaye Dembele Christelle Thibault-Carpentier

PROVIDER: E-GEOD-22137 | ArrayExpress | 2012-03-27

SECONDARY ACCESSION(S): GSE22137PRJNA127575

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome profile reveals AMPA receptor dysfunction in the hippocampus of the Rsk2-knockout mice, an animal model of Coffin-Lowry syndrome.

Mehmood Tahir T Schneider Anne A Sibille Jérémie J Marques Pereira Patricia P Pannetier Solange S Ammar Mohamed Raafet MR Dembele Doulaye D Thibault-Carpentier Christelle C Rouach Nathalie N Hanauer André A

Human genetics 20101130 3

Coffin-Lowry syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes RSK2, a serine/threonine kinase acting in the MAPK/ERK pathway. The mouse invalidated for the Rps6ka3 (Rsk2-KO) gene displays learning and long-term spatial memory deficits. In the current study, we compared hippocampal gene expression profiles from Rsk2-KO and normal littermate mice to identify changes in molecular pathways. Differential expressi ...[more]

PMID: 21116650

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