Transcriptomics

Dataset Information

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Expression profiles of the hippocampus of Rsk2-KO mice


ABSTRACT: Coffin–Lowry Syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes Rsk2, a serine/threonine kinase involved in spatial memory. We analyzed hippocampal gene expression profiles in Rsk2-KO mice to identify changes in molecular pathways. Total RNA was extracted from hippocampi from 6 KO and 6 WT (littermates) 2-month-old male mice. For each genotype, equivalent amounts of RNA from 2 mice were pooled and processed for hybridization to the genome wide oligonucleotide microarray (Murine 430A 2.0 Affymetrix, 22.000 probe sets). Thus, 3 independent pooled samples were hybridized for each genotype. We compared hippocampal gene expression profiles from rsk2-KO and normal littermate mice to identify changes in molecular pathways

ORGANISM(S): Mus musculus  

SUBMITTER: André Hanauer   Nathalie Rouach  Tahir Mehmood  Mohamed Ammar  Patricia Marques-Pereira  Jérémie Sibillec  Anne Schneider  Solange Pannetier  Doulaye Dembele  Christelle Thibault-Carpentier 

PROVIDER: E-GEOD-22137 | ArrayExpress | 2010-12-22

SECONDARY ACCESSION(S): GSE22137PRJNA127575

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome profile reveals AMPA receptor dysfunction in the hippocampus of the Rsk2-knockout mice, an animal model of Coffin-Lowry syndrome.

Mehmood Tahir T   Schneider Anne A   Sibille Jérémie J   Marques Pereira Patricia P   Pannetier Solange S   Ammar Mohamed Raafet MR   Dembele Doulaye D   Thibault-Carpentier Christelle C   Rouach Nathalie N   Hanauer André A  

Human genetics 20101130 3


Coffin-Lowry syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes RSK2, a serine/threonine kinase acting in the MAPK/ERK pathway. The mouse invalidated for the Rps6ka3 (Rsk2-KO) gene displays learning and long-term spatial memory deficits. In the current study, we compared hippocampal gene expression profiles from Rsk2-KO and normal littermate mice to identify changes in molecular pathways. Differential expressi  ...[more]

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