Dataset Information


Transcriptomic profiling of Salmonella during lag, exponential and stationary phases of growth

ABSTRACT: Transcriptional profiles during all phases of growth of Salmonella Typhimurium SL1344 were obtained and used to investigate growth phase-dependent alterations in gene expression. Viable count growth curve analysis of the growth system showed a culture lag time of 2.09 hours, and profiles at 4, 20, 40, 60, 90 and 120 minutes were measured accordingly. For comparison, profiles of the inoculum (0 min), mid-exponential (380 min), late-exponential (630 min), early-stationary (900 min) and late-stationary phase (2880 min) were also obtained. Large-scale gene-expression changes were seen, with substantial changes within 4 minutes of inoculation, indicating the speed and sophistication at which Salmonella senses its new environment during lag phase. Timecourse; 46 samples (11 timepoints, each with two biological replicates and at least two technical replicates); each sample hybridised in a two-channel hybridization against Salmonella genomic DNA as the comparator/reference, which also acted as a control for spot quality.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344  

SUBMITTER: Matthew D Rolfe   R P Betts  C Pin  A D Cameron  M D Rolfe  C J Rice  M Alston  J C Hinton  A Thompson  S Lucchini  M F Stringer  J Baranyi  M W Peck 

PROVIDER: E-GEOD-22294 | ArrayExpress | 2011-12-13



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Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation.

Rolfe Matthew D MD   Rice Christopher J CJ   Lucchini Sacha S   Pin Carmen C   Thompson Arthur A   Cameron Andrew D S AD   Alston Mark M   Stringer Michael F MF   Betts Roy P RP   Baranyi József J   Peck Michael W MW   Hinton Jay C D JC  

Journal of bacteriology 20111202 3

Lag phase represents the earliest and most poorly understood stage of the bacterial growth cycle. We developed a reproducible experimental system and conducted functional genomic and physiological analyses of a 2-h lag phase in Salmonella enterica serovar Typhimurium. Adaptation began within 4 min of inoculation into fresh LB medium with the transient expression of genes involved in phosphate uptake. The main lag-phase transcriptional program initiated at 20 min with the upregulation of 945 gene  ...[more]

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