Transcriptomics

Dataset Information

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PBRM1 Knockdown in RCC Cell Lines


ABSTRACT: PBRM1 was found to be mutated in a high percentage of clear cell RCCs. We performed knockdown of PBRM1 via siRNA and compared with scrambled control in three different RCC cell lines. PBRM1 siRNA and mock treated cell lines were normalized together with 'hypoxic' clear cell renal tumors and normal renal tissue samples from GSE17818.

ORGANISM(S): Homo sapiens  

SUBMITTER: Karl Dykema  

PROVIDER: E-GEOD-22316 | ArrayExpress | 2011-01-20

SECONDARY ACCESSION(S): GSE22316PRJNA127545

REPOSITORIES: GEO, ArrayExpress

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Publications

Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma.

Varela Ignacio I   Tarpey Patrick P   Raine Keiran K   Huang Dachuan D   Ong Choon Kiat CK   Stephens Philip P   Davies Helen H   Jones David D   Lin Meng-Lay ML   Teague Jon J   Bignell Graham G   Butler Adam A   Cho Juok J   Dalgliesh Gillian L GL   Galappaththige Danushka D   Greenman Chris C   Hardy Claire C   Jia Mingming M   Latimer Calli C   Lau King Wai KW   Marshall John J   McLaren Stuart S   Menzies Andrew A   Mudie Laura L   Stebbings Lucy L   Largaespada David A DA   Wessels L F A LF   Richard Stephane S   Kahnoski Richard J RJ   Anema John J   Tuveson David A DA   Perez-Mancera Pedro A PA   Mustonen Ville V   Fischer Andrej A   Adams David J DJ   Rust Alistair A   Chan-on Waraporn W   Subimerb Chutima C   Dykema Karl K   Furge Kyle K   Campbell Peter J PJ   Teh Bin Tean BT   Stratton Michael R MR   Futreal P Andrew PA  

Nature 20110119 7331


The genetics of renal cancer is dominated by inactivation of the VHL tumour suppressor gene in clear cell carcinoma (ccRCC), the commonest histological subtype. A recent large-scale screen of ∼3,500 genes by PCR-based exon re-sequencing identified several new cancer genes in ccRCC including UTX (also known as KDM6A), JARID1C (also known as KDM5C) and SETD2 (ref. 2). These genes encode enzymes that demethylate (UTX, JARID1C) or methylate (SETD2) key lysine residues of histone H3. Modification of  ...[more]

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