Transcriptomics

Dataset Information

5

Transcription profiling by array of lens tissue from mice expressing a dnBrg1 transgenic construct


ABSTRACT: Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific alphaA-crystallin promoter in postmitotic lens fiber cells. Morphological studies revealed abnormal lens fiber cell differentiation in transgenic lenses resulting in cataract. Electron microscopic studies showed abnormal lens suture formation and incomplete karyolysis (denucleation) of lens fiber cells. To identify genes regulated by Brg1, RNA expression profiling was performed in E15.5 embryonic wild type and dnBrg1 transgenic lenses. In addition, comparisons between differentially expressed genes in dnBrg1 transgenic, Pax6 heterozygous, and Hsf4 homozygous lenses identified multiple genes co-regulated by Brg1, Hsf4 and Pax6. Among them DNase IIbeta, a key enzyme required for lens fiber cell denucleation, was found downregulated in each of the Pax6, Brg1 and Hsf4 model systems. Lens-specific deletion of Brg1 using conditional gene targeting demonstrated that Brg1 was required for lens fiber cell differentiation and indirectly for retinal development but was not essential for lens lineage formation. Wild type and dnBrg1 transgenic lenses, 4 biological replicates each

REANALYSED by: E-GEOD-22322

ORGANISM(S): Mus musculus  

SUBMITTER: Akira Nakai   Melinda Pirity  Shuying He  Kveta Cveklova  Ales Cvekl  Ernest R Tamm  Louise Wolf  Bharesh K Chauhan  Ruth Ashery-Padan  Wei-Lin Wang  Jiri Zavadil 

PROVIDER: E-GEOD-22322 | ArrayExpress | 2011-01-01

SECONDARY ACCESSION(S): GSE22322PRJNA141705

REPOSITORIES: GEO, ArrayExpress

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Publications

Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and its denucleation.

He Shuying S   Pirity Melinda K MK   Wang Wei-Lin WL   Wolf Louise L   Chauhan Bharesh K BK   Cveklova Kveta K   Tamm Ernst R ER   Ashery-Padan Ruth R   Metzger Daniel D   Nakai Akira A   Chambon Pierre P   Zavadil Jiri J   Cvekl Ales A  

Epigenetics & chromatin 20101130 1


Brahma-related gene 1 (Brg1, also known as Smarca4 and Snf2β) encodes an adenosine-5'-triphosphate (ATP)-dependent catalytical subunit of the (switch/sucrose nonfermentable) (SWI/SNF) chromatin remodeling complexes. SWI/SNF complexes are recruited to chromatin through multiple mechanisms, including specific DNA-binding factors (for example, heat shock transcription factor 4 (Hsf4) and paired box gene 6 (Pax6)), chromatin structural proteins (for example, high-mobility group A1 (HMGA1)) and/or ac  ...[more]

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