Dataset Information


T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands

ABSTRACT: Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis. Toluidine blue-stained cryostat sections of embryonic day 12.5 submandibular salivary glands were used for laser microdissection using an LMD system (Leica) with a pulsed UV laser on an upright automated microscope. A total of approximately 500 cells from a region immediately adjacent to a forming cleft or from a peripheral end bud region were excised by the laser and transferred to caps of 0.5 ml PCR tubes. SAGE libraries were constructed from the microdissected cleft and bud epithelia cells. SAGE tags were identified using the National Center for Biotechnology Information (NCBI) tag map set for UniGene.

ORGANISM(S): Mus musculus  

SUBMITTER: Takayoshi Sakai   Takayoshi Sakai 

PROVIDER: E-GEOD-22374 | ArrayExpress | 2010-06-16



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Btbd7 regulates epithelial cell dynamics and branching morphogenesis.

Onodera Tomohiro T   Sakai Takayoshi T   Hsu Jeff Chi-feng JC   Matsumoto Kazue K   Chiorini John A JA   Yamada Kenneth M KM  

Science (New York, N.Y.) 20100701 5991

During embryonic development, many organs form by extensive branching of epithelia through the formation of clefts and buds. In cleft formation, buds are delineated by the conversion of epithelial cell-cell adhesions to cell-matrix adhesions, but the mechanisms of cleft formation are not clear. We have identified Btbd7 as a dynamic regulator of branching morphogenesis. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation through its highly focal expression lea  ...[more]

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