Dataset Information


Time course of oxidative stress responses in yeast, caused by Menadione or hydrogen peroxide

ABSTRACT: Oxidative stress caused by Menadione or Hydrogen peroxide in synchronized Saccharomyces cerevisiae cultures. Alpha factor synchronized cultures (0.2-0.4 OD), treated at the beginning of S phase (25 min after release from G1 arrest) with either 2 mM Menadione (MD) or 0.24 mM Hydrogen peroxide (HP), show cell cycle effects. Cells treated with MD arrested at G1. Cells treated with HP delayed at S and then, after removal of HP at 135 minutes , continued the cell cycle, only to arrest at G2/M. Growth was carried out in 30C with shaking (295 rpm). Two time course experiments were performed with each oxidative stress agent, designated as H2O2 and H2O2_II, MD and MD_II. Keywords = oxidative stress Keywords = menadione Keywords = hydrogen peroxide Keywords = time course Keywords = cell cycle Keywords = yeast

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Michael Shapira   David Botstein  Eran Segal 

PROVIDER: E-GEOD-2239 | ArrayExpress | 2005-02-04



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Disruption of yeast forkhead-associated cell cycle transcription by oxidative stress.

Shapira Michael M   Segal Eran E   Botstein David D  

Molecular biology of the cell 20040915 12

The effects of oxidative stress on yeast cell cycle depend on the stress-exerting agent. We studied the effects of two oxidative stress agents, hydrogen peroxide (HP) and the superoxide-generating agent menadione (MD). We found that two small coexpressed groups of genes regulated by the Mcm1-Fkh2-Ndd1 transcription regulatory complex are sufficient to account for the difference in the effects of HP and MD on the progress of the cell cycle, namely, G1 arrest with MD and an S phase delay followed  ...[more]

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