Transcriptomics

Dataset Information

5

Combined miRNA and mRNA analysis of CD133(+) and CD34(+) cells


ABSTRACT: Microarray and miRNA analysis of CD133(+), CD34(+)CD133(-) and CD133(-)CD34(-) cells The goal of the experiment was the comparison of expression levels of mRNA and miRNA in CD133(+) and CD34(+)CD133(-) cells The RNA of CD133(+), CD34(+)CD133(-) and CD133(-)CD34(-) bone marrow cells was amplified by using the µMACSTM SuperAmpTM Kit (Miltenyi Biotec).

SUBMITTER: Ute Bissels  

PROVIDER: E-GEOD-22460 | ArrayExpress | 2011-03-14

SECONDARY ACCESSION(S): GSE22460PRJNA128605

REPOSITORIES: GEO, ArrayExpress

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Publications

Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells.

Bissels Ute U   Wild Stefan S   Tomiuk Stefan S   Hafner Markus M   Scheel Hartmut H   Mihailovic Aleksandra A   Choi Yeong-Hoon YH   Tuschl Thomas T   Bosio Andreas A  

Stem cells (Dayton, Ohio) 20110501 5


MicroRNAs (miRNAs) have been shown to play an important role in hematopoiesis. To elucidate the role of miRNAs in the early steps of hematopoiesis, we directly compared donor-matched CD133(+) cells with the more differentiated CD34(+) CD133(-) and CD34(-) CD133(-) cells from bone marrow on the miRNA and mRNA level. Using quantitative whole genome miRNA microarray and sequencing-based profiling, we found that between 109 (CD133(+) ) and 216 (CD34(-) CD133(-) ) miRNAs were expressed. Quantificatio  ...[more]

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