Transcriptomics

Dataset Information

30

Transcription profile of T. thermophilus HB8 strain lacking mutS, mutL, or mutS2 gene


ABSTRACT: We analyzed the expression profile of mutS, mutL, or mutS2 deletion mutant strain of Thermus thermophils HB8 during the exponential growth phase. When compared with wild type using t-test (P < 0.01), 8, 111, and 18 genes were over 2-fold up-regulated in mutS, mutL, and mutS2-lacking strains, respectively. Keywords: cell type comparison Wild-type and the mutant strains were precultured in 3 mL of rich medium for two times at 70°C and then subcultured to 1000 mL of rich medium. These cells were cultured at70°C for 300 min , and then cells were harvested from 50 ml of the culture. Total RNA were extracted from wild-type and mutant strains and used for the cDNA synthesis by SuperScript II (Invitrogen, Carlsbad, CA). The cDNA fragments were labeled with GeneChip DNA Labeling Reagent (Affymetrix, Santa Clara, CA), using terminal transferase according to the manufacturer’s instructions. The 3’-terminal-labeled cDNA was hybridized with a TTHB8401a520105F GeneChip (Affymetrix) which contained probe sets of 25-mer oligonucleotides for 2238 ORFs and 1096 intergenic regions. After washing and staining with streptavidin-phycoerythrin (Invitrogen) by GeneChip Fluidics Station 450XP (Affymetrix), the array was scanned by a GeneChip Scanner 3000 (Affymetrix). The image data was scaled to the target intensity by one-step Tukey’s biweight algorithm using GeneChip Operating software, version 1.2 (Affymetrix). The data analysis was performed on the Subio platform version 1.6 (Subio Inc., Tokyo, Japan). The genes which had detection call of “presence” more than 4 times from 8 samples were used for following analysis. The normalized intensities for the mutant strains were calculated by using the intensities for wild-type strains of each data set, and then, the average of these intensities were used. The genes which produced P value < 0.01 in the Student’s t-test were extracted. Among them, the genes whose expression level was different more than 2 fold between two strains were considered as significant. For the biological replication, above experiments were performed three times independently.

ORGANISM(S): Thermus thermophilus HB8  

SUBMITTER: Seiki Kuramitsu  Akeo Shinkai   Yoshihiro Agari   Kenji Fukui    

PROVIDER: E-GEOD-22567 | ArrayExpress| 2013-01-27

SECONDARY ACCESSION(S): GSE22567PRJNA128433

REPOSITORIES: GEO, ArrayExpress

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Publications

Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes.

Fukui Kenji K   Wakamatsu Taisuke T   Agari Yoshihiro Y   Masui Ryoji R   Kuramitsu Seiki S  

PloS one 20110428 4


Oxidative stress generates harmful reactive oxygen species (ROS) that attack biomolecules including DNA. In living cells, there are several mechanisms for detoxifying ROS and repairing oxidatively-damaged DNA. In this study, transcriptomic analyses clarified that disruption of DNA repair genes mutS and mutL, or the anti-recombination gene mutS2, in Thermus thermophilus HB8, induces the biosynthesis pathway for vitamin B(1), which can serve as an ROS scavenger. In addition, disruption of mutS, mu  ...[more]

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