Transcriptomics

Dataset Information

0

Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation


ABSTRACT: Background: Type I interferons (IFNs) are essential to the clearance of viral diseases, in part by initiating upregulation of IFN regulated genes (IRGs). A clear distinction between genes upregulated directly by virus and genes upregulated by secondary IFN production has not been made. Here we investigated the genes regulated by IFN-a2b compared to the genes regulated by SARS-CoV infection in ferrets. Methods: We characterized early host immune responses in peripheral blood and lung necropsies of ferrets injected with IFN-a2b or infected with SARS-CoV/Tor 2 strain, using microarray analysis on the Affymetrix platform. Results: We identified a common IRG signature that was upregulated in both SARS-CoV infected ferrets as well as in ferrets injected with IFN-a2b. We also identified unique patterns of gene expression for leukocyte activation, cell adhesion and complement pathways between IFN-a2b injection and SARS-CoV infection. Conclusions: Our results define the effects of IFN-a2b on the immune system of ferrets highlighting genes regulated by IFN during SARS-CoV infection. We have shown the similarities and differences of top funcional gene groups as well as pathways that play key roles in early immune responses in ferrets in response to IFN-a2b or SARS-CoV. Key words: ferret, gene expression, SARS, interferon. Keywords: time course In experiments with IFN-a2b, for peripheral blood, 15 ferrets were randomly allocated to 3 groups: Day 0, 5 ferrets (no IFN injection), day 1, 6 ferrets (injected), and day 2, 4 ferrets (injected). For lung necropsies of injected ferrets with IFN-a2b, we used 12 ferrets in 3 groups: 4 ferrets, day 0 (no IFN injection), 4 ferrets, day 1 (injected) and 4 ferrets, day 2 (injected). Experimental groups for SARS-CoV infection was as follows: For peripheral blood, 3 and 4 ferrets for day 0 (no infection) and day 2 (infection) respectively. For lung neceropsies, a total of 9 ferrets in 3 groups, each with 3 replicates for day 0 (no infection), day 1 (infection) and day 2 (infection).

ORGANISM(S): Mustela putorius furo  

SUBMITTER: Longsi Ran  David J Kelvin    

PROVIDER: E-GEOD-22581 | ArrayExpress | 2010-12-01

SECONDARY ACCESSION(S): GSE22581PRJNA128405

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation.

Danesh Ali A   Cameron Cheryl M CM   León Alberto J AJ   Ran Longsi L   Xu Luoling L   Fang Yuan Y   Kelvin Alyson A AA   Rowe Thomas T   Chen Honglin H   Guan Yi Y   Jonsson Colleen B CB   Cameron Mark J MJ   Kelvin David J DJ  

Virology 20101028 1


Type I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), ch  ...[more]

Similar Datasets

2010-12-01 | GSE22581 | GEO
2013-05-28 | E-GEOD-11704 | ArrayExpress
| GSE11704 | GEO
| GSE19137 | GEO
2010-02-01 | GSE17400 | GEO
2013-01-28 | E-GEOD-36019 | ArrayExpress
2013-09-06 | E-GEOD-47957 | ArrayExpress
2010-02-24 | E-GEOD-17400 | ArrayExpress
2014-09-19 | E-GEOD-17400 | ExpressionAtlas
2010-04-12 | E-GEOD-17079 | ArrayExpress