Dataset Information


Plasmodium falciparum W2/Dd2 Day 8 gametocytes

ABSTRACT: The sexual stages are vital phases in malaria parasite transmission and are the targets of various interventions such as transmission blocking vaccines. The molecular mechanisms underlying sexual development, however, remain poorly understood. We report mappping of a determinant previously linked to a male gametocyte development defect in the P. falciparum Dd2 parasite to an 82 kb region on chromosome 12. In order to find a critical gene in this region, we compared gene expression pattern in sexual stage of the parasite between Dd2 and its normal gametocyte-producing ancestor W2 clones. The region contains a sexual stage specific gene (pfmdv 1) that is expressed substantially at a lower level in the Dd2 than in W2 parasite. Disruption of pfmdv 1 results in a dramatic reduction in mature gametocytes, especially male gametocytes, with the majority of sexually committed parasites arrested at stage-I. The pfmdv-1 knockout parasites show an enlarged nucleus, often with separation of the inner and outer nuclear membranes and presence of multi-membrane vesicles in red blood cell cytoplasm. Mosquito infectivity of the knockout parasites is also greatly reduced, but not completely lost, suggesting presence of compensatory mechanisms in the sexual development pathways. Data include Day 8 gametocytes of male defective Dd2 and parental W2 clones of Plasmodium falciparum. The series includes three biological repeats. Keywords: repeat sample

ORGANISM(S): Plasmodium falciparum  

SUBMITTER: Tetsuya Furuya   Xin-zhuan Su 

PROVIDER: E-GEOD-2265 | ArrayExpress | 2010-06-10



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Disruption of a Plasmodium falciparum gene linked to male sexual development causes early arrest in gametocytogenesis.

Furuya Tetsuya T   Mu Jianbing J   Hayton Karen K   Liu Anna A   Duan Junhui J   Nkrumah Louis L   Joy Deirdre A DA   Fidock David A DA   Fujioka Hisashi H   Vaidya Akhil B AB   Wellems Thomas E TE   Su Xin-zhuan XZ  

Proceedings of the National Academy of Sciences of the United States of America 20051107 46

A male gametocyte defect in the Plasmodium falciparum Dd2 parasite was previously discovered through the observation that all progeny clones in a Dd2 x HB3 genetic cross were the result of fertilization events between Dd2 female and HB3 male gametes. A determinant linked to the defect in Dd2 was subsequently mapped to an 800-kb segment on chromosome 12. Here, we report further mapping of the determinant to an 82-kb region and the identification of a candidate gene, P. falciparum male development  ...[more]

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