Dataset Information


Expression data from human PBMC induced by mixed Congeners of PCBs at Human equivalence

ABSTRACT: Exposure to Polychlorobiphenyls (PCBs) is known to cause serious health effects in human but the gene expression profiles leading to development of different diseases and disorders are not fully understood. The knowledge of global gene expression will help us to develop early disease or disorder biomarkers for PCB induced health effects. We used microarrays to detail the global gene expression profile underlying the effects of mixed PCB exposure at Slovak Human equivalance Level on human PBMC leading to identification of distinct classes of up-regulated and down-regulated genes and cellular processes. PBMC cells are grown for 48 hours in RPMI 1640 supplemented with 10% FBS and 1X Pennicilllin-Streptromycin with 1.25 microgram/ml PHA-M and 0.15% (v/v)pokeweed mitogen and 50 microgram beta-mercaptoethanol. Trizol extraction of total RNA was performed according to the manufacturer's instructions. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HGU133 plus 2.0). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneArray Scanner 3000. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

ORGANISM(S): Homo sapiens  

SUBMITTER: Dean Sonneborn   Irva Hertz-Picciotto  Partha Mitra  Lubica Pulkovicova  Sisir K. Dutta  Somiranjan Ghosh  Eric P Hoffman  Sisir K Dutta  Tomas Trnovec  Svetlana Ghimbovschi 

PROVIDER: E-GEOD-22668 | ArrayExpress | 2015-06-22



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Transcriptional profiling and biological pathway analysis of human equivalence PCB exposure in vitro: indicator of disease and disorder development in humans.

Ghosh Somiranjan S   Mitra Partha S PS   Loffredo Christopher A CA   Trnovec Tomas T   Murinova Lubica L   Sovcikova Eva E   Ghimbovschi Svetlana S   Zang Shizhu S   Hoffman Eric P EP   Dutta Sisir K SK  

Environmental research 20150227

Our earlier gene-expression studies with a Slovak PCBs-exposed population have revealed possible disease and disorder development in accordance with epidemiological studies. The present investigation aimed to develop an in vitro model system that can provide an indication of disrupted biological pathways associated with developing future diseases, well in advance of the clinical manifestations that may take years to appear in the actual human exposure scenario.We used human Primary Blood Mononuc  ...[more]

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