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Omics score: 5

Saccharomyces cerevisiae grown in nitrogen depletion with MTP priming

ABSTRACT: Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Saccharomyces cerevisiae S288c was grown on two different media.

OTHER RELATED OMICS DATASETS IN: PRJNA133085PRJNA133083PRJNA129907PRJNA133081PRJNA133099

ORGANISM(S): Saccharomyces Cerevisiae

SUBMITTER: Jeffrey P Townsend Francesc Lopez

PROVIDER: E-GEOD-22936 | ArrayExpress | 2010-07-17

SECONDARY ACCESSION(S): GSE22936PRJNA133099

REPOSITORIES: GEO, ArrayExpress

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			Action	DRS
	E-GEOD-22936.README.txt	Txt
	E-GEOD-22936.idf.txt	Idf
	E-GEOD-22936.processed.1.zip	Processed
	E-GEOD-22936.raw.1.zip	Raw
	E-GEOD-22936.sdrf.txt	Txt

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Publications

Multi-targeted priming for genome-wide gene expression assays.

Adomas Aleksandra B AB Lopez-Giraldez Francesc F Clark Travis A TA Wang Zheng Z Townsend Jeffrey P JP

BMC genomics 20100817

<h4>Background</h4>Complementary approaches to assaying global gene expression are needed to assess gene expression in regions that are poorly assayed by current methodologies. A key component of nearly all gene expression assays is the reverse transcription of transcribed sequences that has traditionally been performed by priming the poly-A tails on many of the transcribed genes in eukaryotes with oligo-dT, or by priming RNA indiscriminately with random hexamers. We designed an algorithm to fin ...[more]

PMID: 20716356

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