Transcriptomics

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Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo wild-type mouse model.


ABSTRACT: Ischemic stroke triggers severe focal hypoperfusion accompanied with deprivation of oxygen and glucose to the cerebral tissue, together with loss of ATP, depolorization of neurons, elevated extracellular potassium concentration, and subsequently leads to excitotoxicity as well as increased oxidative stress promoting microvascular injury, blood-brain-barrier deregulation, post-ischemic inflammation and eventually the consequential neurological deficit. Although reperfusion of ischemic brain tissue is critical for restoring normal function, it can paradoxically result in secondary damage, called ischemia/reperfusion (I/R) injury. Microarray analysis was performed on the right striatum and cortex (corresponded to infarct area) of post-I/R injured brain tissues of wild-type (WT-MCAO) using Illumina mouse Ref8 V2 genechips. Suture-induced middle cerebral artery occlusion was induced for 2h followed by reperfusion, with tissue extraction taking place 2h, 8h and 24h post-reperfusion (n=4 respectively). Sham controls were included in this study too (n=4 respectively).

ORGANISM(S): Mus musculus  

SUBMITTER: Nam S Cheung  Jayapal Manikandan   Peter Crack   Minghui J Chen   Minghui Jessica Chen    

PROVIDER: E-GEOD-23160 | ArrayExpress | 2011-01-10

SECONDARY ACCESSION(S): GSE23160PRJNA133269

REPOSITORIES: GEO, ArrayExpress

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Publications

A global transcriptomic view of the multifaceted role of glutathione peroxidase-1 in cerebral ischemic-reperfusion injury.

Chen Minghui Jessica MJ   Wong Connie H Y CH   Peng Zhao Feng ZF   Manikandan Jayapal J   Melendez Alirio J AJ   Tan Theresa M TM   Crack Peter J PJ   Cheung Nam Sang NS  

Free radical biology & medicine 20101228 6


Transient cerebral ischemia often results in secondary ischemic/reperfusion injury, the pathogenesis of which remains unclear. This study provides a comprehensive, temporal description of the molecular events contributing to neuronal injury after transient cerebral ischemia. Intraluminal middle cerebral artery occlusion (MCAO) was performed to induce a 2-h ischemia with reperfusion. Microarray analysis was then performed on the infarct cortex of wild-type (WT) and glutathione peroxidase-1 (a maj  ...[more]

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