Project description:au10-07_mpk4gof - mpk4/mpk4 gof vs mpk4/mpk4 vs col0 - Genes regulated by MPK4 pathway. - In order to study of the genes controlled by MPK4 pathway we generate mpk4 complemented lines expressing active MPK4 (MPK4 GOF). For the transcriptome analysis we compare the MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0. Keywords: genotype comparaison 6 dye-swap - CATMA arrays Comparisons of MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0.
Project description:inra12-05_mut_flg_ii-FLG-MPKs ra12-05_mut_flg_ii - inra12-05_mut_flg_ii - Analysis of flg22-induced transcriptional changes in mpk3, mpk4 and mpk6 mutants. - 2 weeks old seedlings compared for transcriptome after a 30min mock or 1µM flg22 treatment. 21 dye-swap - gene knock out,normal vs mutant comparison,treated vs untreated comparison
Project description:rs06-06_mirna - flg22 time course - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin derived peptide) over a one hour timecourse. Keywords: time course,treated vs untreated comparison 7 dye-swap - CATMA arrays
Project description:au10-06_salmonella - salmonell inva - Transcriptomic analysis between Salmonella wt and differents mutants in type3 secretion system. - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h. at 9 am samonella was added to a final concentration of OD600=0.2 2 dye-swap - normal vs antisense mutant comparison
Project description:au10-01_overexpressionoxi1 - au10-01_overexpressionoxi1 - Analysis of the overexpression of the Oxi1 - Comparision between the overexpression of oxi1 and wt. 2 dye-swap - normal vs transgenic comparison