Transcriptomics

Dataset Information

7

Gene Expression Profiles in Immature and In vitro matured bovine Oocytes


ABSTRACT: In cattle, almost all fully grown vesicle stage oocytes (GV) have the ability to resume meisos, develop to Metaphase II stage (MII), support fertilization and progress through the early embryonic cycles in vitro. Yet without intensive selection, the majority fail to develop to the blastocyst stage. Using the Affymetrix Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of the bovine oocyte and to identify the key pathways associated with oocyte meiotic maturation and developmental potential. Immature and in vitro matured bovine oocytes were collected for RNA extraction and hybridization on Affymetrix GeneChip Bovine Genome array. Careful removal of cumulus and selection of oocytes was carried out under the stereo microscope in order to examine the actual cumulus-free temporal oocyte gene expression profiles. Immature oocytes at time 0 h and in vitro matured oocytes at 24 h were collected for analysis.

ORGANISM(S): Bos taurus  

SUBMITTER: Solomon Mamo   Claudia L Leal  Fiona Carter  Paul McGettigan  Abdullah A Naib  Trudee Fair  Pat Lonergan 

PROVIDER: E-GEOD-23449 | ArrayExpress | 2011-04-25

SECONDARY ACCESSION(S): GSE23449PRJNA130999

REPOSITORIES: GEO, ArrayExpress

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Publications

Sequential analysis of global gene expression profiles in immature and in vitro matured bovine oocytes: potential molecular markers of oocyte maturation.

Mamo Solomon S   Carter Fiona F   Lonergan Patrick P   Leal Cláudia Lv CL   Al Naib Abdullah A   McGettigan Paul P   Mehta Jai P JP   Evans Alexander Co AC   Fair Trudee T  

BMC genomics 20110316


BACKGROUND: Without intensive selection, the majority of bovine oocytes submitted to in vitro embryo production (IVP) fail to develop to the blastocyst stage. This is attributed partly to their maturation status and competences. Using the Affymetrix GeneChip Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of bovine oocytes and then use a variety of approaches to determine whether the  ...[more]

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