Transcriptomics

Dataset Information

3

Light/dark- and temperature-regulated transcriptional rhythms in adult Caenorhabditis elegans


ABSTRACT: Most organisms have an endogenous circadian clock that is synchronized to environmental signals such as light and temperature. Although circadian rhythms have been described in the nematode C. elegans at the behavioral level, these rhythms appear to be relatively non-robust. Moreover, in contrast to other animal models, no circadian transcriptional rhythms have been identified. Thus, whether this simple nematode contains a bona fide circadian clock remains an open question. We used microarray experiments to identify light- and temperature-regulated transcriptional rhythms in C. elegans, and show that subsets of these transcripts are regulated in a circadian manner. In addition, we find that light and temperature also globally drive the expression of many genes, indicating that C. elegans exhibits systemic responses to these stimuli. Populations of growth-synchronized wild-type C. elegans L1 larvae were entrained for 5 days until adulthood to 12:12 hr light/dark (LD) cycles (500-1000 lux) at a constant temperature of 18°C, or for 4 days to 12:12 hr temperature cycles (25:15°C - warm/cold or WC) in constant darkness. RNA was collected every 4 hrs during the last entrainment and the subsequent free-running days and analyzed via hybridization of Affymetrix GeneChips. L4 larvae were transferred to FUDR-containing plates to inhibit embryonic development.

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Alexander M van der Linden   Sara Wasserman  Sebastian Kadener  Matthew Beverly  Alexander Martinus van der Linden  Piali Sengupta  Joseph Rodriguez  Michael Rosbash 

PROVIDER: E-GEOD-23528 | ArrayExpress | 2010-08-31

SECONDARY ACCESSION(S): GSE23528PRJNA131785

REPOSITORIES: GEO, ArrayExpress

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