Transcriptomics

Dataset Information

2

Conversion of mouse fibroblast to hepatocyte-like cells by defined factors


ABSTRACT: Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (Hnf1alpha, Gata4, and Foxa3) and p19Arf inactivation, tail-tip fibroblasts can be converted to hepatocyte-like (iHep) cells, expressing hepatocyte marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo. p19Arf-null TTFs were tranfected with 3 liver enriched transcription factors, then changed to modified Block's medium. To enrich iHep cells, epithelial cells were enriched by partial trypsin digestion.

ORGANISM(S): Mus musculus  

SUBMITTER: Pengyu Huang   Lijian Hui 

PROVIDER: E-GEOD-23635 | ArrayExpress | 2011-05-11

SECONDARY ACCESSION(S): GSE23635PRJNA130939

REPOSITORIES: GEO, ArrayExpress

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