Transcriptomics

Dataset Information

3

Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”*


ABSTRACT: Lactobacillus helveticus is a rod-shaped lactic acid bacterium that is widely used in the manufacture of fermented dairy foods and for production of bioactive peptides from milk proteins. Although L. helveticus is commonly associated with milk environments, phylogenetic studies show it is closely related to an intestinal species, Lactobacillus acidophilus, which has been shown to impart probiotic health benefits to humans. This relationship has fueled a prevailing hypothesis that L. helveticus is a highly specialized derivative of L. acidophilus which has adapted to acidified whey. However, L. helveticus has also been sporadically recovered from non-dairy environments, which argues the species may not be as highly specialized as is widely believed. This study employed genome sequence analysis and comparative genome hybridizations to investigate genomic diversity among L. helveticus strains collected from cheese, whey, and whiskey malt, as well as commercial cultures used in manufacture of cheese or bioactive dairy foods. Results revealed considerable variability in gene content between some L. helveticus strains, and indicated the species should not be viewed as a strict dairy-niche specialist. In addition, comparative genomic analyses provided new insight on several industrially and ecologically important attributes of L. helveticus that may facilitate commercial strain selection. 42 samples were hybridized to the microarray chip, which contains probe sequences from L. helveticus CNRZ32. CNRZ32 was also hybridized and used as the reference sample. Data from the microarray was statistically analyzed using the R software. Samples were compared to the reference (CNRZ32) to investigate genome diversity amoung L. helveticus strains,

ORGANISM(S): Lactobacillus helveticus  

SUBMITTER: Erasmo Neviani   Vanessa de Carvalho  James L Steele  Rebecca L Larsen  Thomas Tompkins  Jeff Broadbent  Monica Gatti  Finn Vogensen  Joanne Hughes  Ylva Ardö  Marcin Swiatek  Dennis L Welker  Hui Cai  Angela De Lorentiis  Guillaume Barreau  Jeff R Broadbent 

PROVIDER: E-GEOD-23660 | ArrayExpress | 2013-08-16

SECONDARY ACCESSION(S): GSE23660PRJNA130859

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-23660.idf.txt Idf
E-GEOD-23660.processed.1.zip Processed
E-GEOD-23660.raw.1.zip Raw
E-GEOD-23660.sdrf.txt Txt
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