Transcriptomics

Dataset Information

4

Interior Spruce_Bark_PissodesStrobi _Herbivory


ABSTRACT: We performed a transcriptome analysis of interior spruce (Picea glauca x engelmannii) bark response to weevil (Pissodes strobi) feeding using 21.8K spruce microarray (that contains 21.8 thousand unique transcripts). This microarray study revealed a large rearrangement of the interior spruce bark transcriptome in response to weevil feeding involving differential expression of close to 20% of the studied transcriptome. RNA was isolated from the bark of interior spruce exposed to weevil feeding and from the bark of untreated trees at three time points (6 hours, 2 days and 2 weeks). Four independent biological replicates were included for treatment and control at each time point. Four hybridizations were performed for treatment and control comparison within each time point (6 hours, 2 days, 2 weeks) and one hybridization was performed for each comparison between time points for both treatment and control (total 18 hybridizations/slides).

ORGANISM(S): Picea engelmannii x Picea glauca  

SUBMITTER: Joerg Bohlmann   Rick White  Colette Breuil  Natalia Kolosova  Steven Ralph 

PROVIDER: E-GEOD-23715 | ArrayExpress | 2012-12-01

SECONDARY ACCESSION(S): GSE23715PRJNA130881

REPOSITORIES: GEO, ArrayExpress

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