Genomics

Dataset Information

299

Dynamic BRG1 Recruitment During T Helper Differentiation And Activation Reveals Distal Regulatory Elements


ABSTRACT: We found binding of the remodeling protein BRG1 was programmed by lineage and activation signals. BRG1 binding was positively correlated with gene activity at protein-coding and miRNA genes. BRG1 binding was found at promoters and distal regions, including known and novel distal regulatory elements. Distal BRG1 binding correlated with expression, and novel distal sites possessed enhancer activity, suggesting a general role for BRG1 in long-distance gene regulation. Together, these findings suggest BRG1 interprets differentiation and activation signals and plays a causal role in gene regulation, chromatin structure, and cell fate. Our findings indicate BRG1 binding is a useful marker for identifying cis-regulatory regions in protein-coding and miRNA genes. Compare BRG1 binding in T helper subsets genome wide; Naïve, resting Th1, resting Th2, Stimulated Th1, Stimulated Th2, Stimulated Th17, compared to input DNA as negative control

ORGANISM(S): Mus musculus  

SUBMITTER: William H Wood III   Kevin G Becker  Andrea L Wurster  Patricia Precht  Supriyo De  Michael J Pazin 

PROVIDER: E-GEOD-23719 | ArrayExpress | 2011-03-01

SECONDARY ACCESSION(S): SRP005500GSE23719PRJNA130875

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Dynamic BRG1 recruitment during T helper differentiation and activation reveals distal regulatory elements.

De Supriyo S   Wurster Andrea L AL   Precht Patricia P   Wood William H WH   Becker Kevin G KG   Pazin Michael J MJ  

Molecular and cellular biology 20110124 7


T helper cell differentiation and activation require specific transcriptional programs accompanied by changes in chromatin structure. However, little is known about the chromatin remodeling enzymes responsible. We performed genome-wide analysis to determine the general principles of BRG1 binding, followed by analysis of specific genes to determine whether these general rules were typical of key T cell genes. We found that binding of the remodeling protein BRG1 was programmed by both lineage and  ...[more]

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