Transcriptomics

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Genes modulated by rhBMP2 and rhBMP7 during osteoblastic differentiation of C2C12 cells


ABSTRACT: This study analyses the gene expression profile of pre-osteoblastic C2C12 mice cells during osteodifferentiation in control and induced by rhBMP2 and rhBMP7 using DNA microarrays. Genes which were already known to be activated by BMP2, to be also activated by rhBMP7, indicating that BMP7 induces these genes in a similar fashion as BMP2. Moreover, we identified some new genes, such as Hoxc8, Glis1, Glis3, Ecsit, LrP6, Dvl1, Grn, BMP1, Ltbp2, Plod1, Htra1 and IGFBP-rP10, to be upregulated by both rhBMPs. Total RNA from mouse pre-myoblastic and pre-osteoblastic C2C12 cell line, after 12h of treatment with rhBMP2 and rhBMP7, was hybridized to a microarray and analyzed.

ORGANISM(S): Mus musculus  

SUBMITTER: Jose M Granjeiro   Maria Angélica Lopes de Souza  Mari C Sogayar  Juan C Bustos-Valenzuela  Andre Fujita 

PROVIDER: E-GEOD-23783 | ArrayExpress | 2011-11-17

SECONDARY ACCESSION(S): GSE23783PRJNA130723

REPOSITORIES: GEO, ArrayExpress

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Publications

Unveiling novel genes upregulated by both rhBMP2 and rhBMP7 during early osteoblastic transdifferentiation of C2C12 cells.

Bustos-Valenzuela Juan C JC   Fujita Andre A   Halcsik Erik E   Granjeiro Jose M JM   Sogayar Mari C MC  

BMC research notes 20110926


FINDINGS: We set out to analyse the gene expression profile of pre-osteoblastic C2C12 cells during osteodifferentiation induced by both rhBMP2 and rhBMP7 using DNA microarrays. Induced and repressed genes were intercepted, resulting in 1,318 induced genes and 704 repressed genes by both rhBMP2 and rhBMP7. We selected and validated, by RT-qPCR, 24 genes which were upregulated by rhBMP2 and rhBMP7; of these, 13 are related to transcription (Runx2, Dlx1, Dlx2, Dlx5, Id1, Id2, Id3, Fkhr1, Osx, Hoxc8  ...[more]

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