Transcriptomics

Dataset Information

3

Progressive lengthening of 3' untranslated regions of mRNAs by alternative polyadenylation during mouse embryonic development


ABSTRACT: The 3' untranslated regions (3' UTRs) of mRNAs contain cis-acting elements for posttranscriptional regulation of gene expression. Here, we report that mouse genes tend to express mRNAs with longer 3' UTRs as embryonic development progresses. This global regulation is controlled by alternative polyadenylation and coordinates with initiation of organogenesis and aspects of embryonic development, including morphogenesis, differentiation, and proliferation. Using myogenesis of C2C12 myoblast cells as a model, we recapitulated this process in vitro and found that 3' UTR lengthening is likely caused by weakening of mRNA polyadenylation activity. Because alternative 3' UTR sequences are typically longer and have higher AU content than constitutive ones, our results suggest that lengthening of 3' UTR can significantly augment posttranscriptional control of gene expression during embryonic development, such as microRNA-mediated regulation. Two biological replicates of C2C12 growth and differentiation conditions, repesctively

ORGANISM(S): Mus musculus  

SUBMITTER: Bin Tian   Zhe Ji 

PROVIDER: E-GEOD-23871 | ArrayExpress | 2010-08-31

SECONDARY ACCESSION(S): GSE23871PRJNA130597

REPOSITORIES: GEO, ArrayExpress

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Publications

Progressive lengthening of 3' untranslated regions of mRNAs by alternative polyadenylation during mouse embryonic development.

Ji Zhe Z   Lee Ju Youn JY   Pan Zhenhua Z   Jiang Bingjun B   Tian Bin B  

Proceedings of the National Academy of Sciences of the United States of America 20090416 17


The 3' untranslated regions (3' UTRs) of mRNAs contain cis-acting elements for posttranscriptional regulation of gene expression. Here, we report that mouse genes tend to express mRNAs with longer 3' UTRs as embryonic development progresses. This global regulation is controlled by alternative polyadenylation and coordinates with initiation of organogenesis and aspects of embryonic development, including morphogenesis, differentiation, and proliferation. Using myogenesis of C2C12 myoblast cells a  ...[more]

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