Transcriptomics

Dataset Information

3

Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.


ABSTRACT: Background: The high number of heavy metal resistance genes in the soil bacterium Cupriavidus metallidurans CH34 makes it an interesting model organism to study microbial responses to heavy metals. Results: In this study the transcriptional response of this bacterium was measured after challenging it to a wide range of sub-lethal concentrations of various essential or toxic metals. Considering the global transcriptional responses for each challenge as well as by identifying the overlap in upregulated genes between different metal responses, the sixteen metals could be clustered in three different groups. Additionally, next to the assessment of the transcriptional response of already known metal resistance genes, new metal response gene clusters were identified. The majority of the metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex cross-talk at transcriptional level between the different metal responses. The highly redundant nature of these metal resistant regions – illustrated by the large number of paralogous genes – combined with the phylogenetic distribution of these metal response regions within evolutionary related and other metal resistant bacteria, provides important insights on the recent evolution of this naturally soil dwelling bacterium towards a highly metal-resistant strain found in harsh and anthropogenic environments. Conclusions: The metal-resistant soil bacterium Cupriavidus metallidurans CH34 displays myriads of gene expression patterns when exposed to a wide range of heavy metals at non-lethal concentrations. The interplay between the different gene expression clusters points towards a complex cross-regulated regulatory network governing heavy metal resistance in C. metallidurans CH34. Keywords: Cupriavidus metallidurans CH34, transcriptional regulation, heavy metal resistance Two-condition experiments. Comparing samples after induction with heavy metals versus non-induced samples. Biological duplicate or triplicate. Each array contains 3 or 4 technical replicates.

ORGANISM(S): Cupriavidus metallidurans CH34  

SUBMITTER: Natalie Leys   Pieter Monsieurs  Paul J Janssen  Max Mergeay  Hugo Moors  Ann Janssen  Rob Van Houdt  Ilse Coninx 

PROVIDER: E-GEOD-23876 | ArrayExpress | 2011-06-28

SECONDARY ACCESSION(S): GSE23876PRJNA130603

REPOSITORIES: GEO, ArrayExpress

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Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.

Monsieurs Pieter P   Moors Hugo H   Van Houdt Rob R   Janssen Paul J PJ   Janssen Ann A   Coninx Ilse I   Mergeay Max M   Leys Natalie N  

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 20110625 6


The soil bacterium Cupriavidus metallidurans CH34 contains a high number of heavy metal resistance genes making it an interesting model organism to study microbial responses to heavy metals. In this study the transcriptional response of strain CH34 was measured when challenged to sub-lethal concentrations of various essential or toxic metals. Based on the global transcriptional responses for each challenge and the overlap in upregulated genes between different metal responses, the sixteen metals  ...[more]

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