Transcriptomics

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Heat stress expression data from developing barley caryopses


ABSTRACT: High temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. In this study we used the 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) caryopses at 12 days post anthesis to 0.5h, 3h and 6h of heat stress exposure. Developing barley caryopses were harvested at different time-points of a day during heat stress (42°C) (2:00 PM, 5:00 PM and 8:00 PM). Heat stress started at 1:30PM. Pooled caryopses (form 3 plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

ORGANISM(S): Hordeum vulgare  

SUBMITTER: Joachim Kilian   Klaus Harter  Dierk Wanke  Elke Mangelsen  Eva Sundberg  Christer Jansson 

PROVIDER: E-GEOD-23896 | ArrayExpress | 2010-11-22

SECONDARY ACCESSION(S): GSE23896PRJNA130435

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome analysis of high-temperature stress in developing barley caryopses: early stress responses and effects on storage compound biosynthesis.

Mangelsen Elke E   Kilian Joachim J   Harter Klaus K   Jansson Christer C   Wanke Dierk D   Sundberg Eva E  

Molecular plant 20101005 1


High-temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. We used the Affymetrix 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) seeds, termed caryopses, after 0.5, 3, and 6 h of heat stress exposure; 958 induced and 1122 repressed genes exhibited spatial and temporal expression patterns that provide a detailed insight into the caryopses' early heat stre  ...[more]

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