Dataset Information


Cloning of 50-75 nt small RNAs associated with Armitage in OSCs

ABSTRACT: Armitage (Armi) is a component of Yb bodies and functions in the primary piRNA processing pathway. Armi interaction with Piwi does not require Yb expression; however, without Yb, the Armi-Piwi complex does not localize at Yb bodies and contains few piR-ILs (small RNAs associated with Armitage complex). These results suggest that only upon its localization at Yb bodies does the Armi-Piwi-Yb complex gain an opportunity to interact with piR-ILs. Currently, however, it remains unclear how piR-ILs are loaded onto the Piwi complex at Yb bodies and which protein(s) within the complex is directly bound to them. 1 sample examined: Ovarian somatic cell (OSC) line

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Mikiko C Siomi   Hirotsugu Ishizu  Haruhiko Siomi  Kuniaki Saito 

PROVIDER: E-GEOD-23931 | ArrayExpress | 2010-10-24



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Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila.

Saito Kuniaki K   Ishizu Hirotsugu H   Komai Miharu M   Kotani Hazuki H   Kawamura Yoshinori Y   Nishida Kazumichi M KM   Siomi Haruhiko H   Siomi Mikiko C MC  

Genes & development 20101021 22

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose tha  ...[more]

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