Transcriptomics

Dataset Information

4

Budding yeast RNA polymerase II CTD phosphorylation and transcription termination factor localization


ABSTRACT: Whole genome analysis of total RNA pol II, Ser2-, Ser5- and Ser7-phosphorylated RNA pol II, in WT and mutants of the C-terminal domain (CTD) kinases Ctk1 and Kin28, and localization of the termination factors Pcf11, Nrd1 and Rat1. ChIP-chip using ligation-mediated PCR-amplified material hybridized to NimbleGen 385K arrays (50mers, median probe spacing 32 bp, cat. No. C4214-00-01).

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Hyunmin Kim   David Bentley  David L. Bentley 

PROVIDER: E-GEOD-23960 | ArrayExpress | 2010-09-15

SECONDARY ACCESSION(S): GSE23960PRJNA130385

REPOSITORIES: GEO, ArrayExpress

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Publications

Gene-specific RNA polymerase II phosphorylation and the CTD code.

Kim Hyunmin H   Erickson Benjamin B   Luo Weifei W   Seward David D   Graber Joel H JH   Pollock David D DD   Megee Paul C PC   Bentley David L DL  

Nature structural & molecular biology 20100912 10


Phosphorylation of the RNA polymerase (Pol) II C-terminal domain (CTD) repeats (1-YSPTSPS-7) is coupled to transcription and may act as a 'code' that controls mRNA synthesis and processing. To examine the code in budding yeast, we mapped genome-wide CTD Ser2, Ser5 and Ser7 phosphorylations and the CTD-associated termination factors Nrd1 and Pcf11. Phospho-CTD dynamics are not scaled to gene length and are gene-specific, with highest Ser5 and Ser7 phosphorylation at the 5' ends of well-expressed  ...[more]

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