Transcriptomics

Dataset Information

5

Transcriptomic profiles of six strains of Lactococcus lactis in ultrafiltration-cheese model


ABSTRACT: The intra sub-species diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in UF-cheese model. Six strains were isolated from various sources, but all are exhibiting a dairy phenotype. Our results showed that, the six strains exhibited small phenotypic differences since similar behaviour in terms of growth was obtained during cheese ripening while only different acidification capability was detected. Even if all strains displayed high genomic similarities, sharing a high core genome of almost two thousands genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences. This strains with the same dairy origin. Strains were cultured on skimmed raw milk ultrafiltration (UF) retentate. The UF retentate was pre-incubated overnight at 4 °C, then 45 minutes at 50 °C and homogenized during 1.5 minutes at 24 000 rpm with an ultra-turax (Imlab, France). After addition of rennet (0.3 µl ml-1), 400 g UF retentate was inoculated at 2 106 CFU/g with L. lactis subsp. lactis strains. After incubation for 8 hours at 30 °C, the cheeses were transferred at 12° C until 7 days for ripening simulation. At least three independent cultures of the six strains were performed. Total RNA was extracted from cells grown 24 hours in UF-cheese and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. 3 independent repetitions were performed.

ORGANISM(S): Lactococcus lactis  

SUBMITTER: Muriel Cocaign-Bousquet   Pascal Loubiere  Tamara Cardoso  Sunthorn Kanchanatawee  Laurence Girbal  Marie-Line Daveran-Mingot  Punthip Tan-a-ram 

PROVIDER: E-GEOD-23987 | ArrayExpress | 2012-09-07

SECONDARY ACCESSION(S): GSE23987PRJNA133193

REPOSITORIES: GEO, ArrayExpress

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Publications

Assessment of the diversity of dairy Lactococcus lactis subsp. lactis isolates by an integrated approach combining phenotypic, genomic, and transcriptomic analyses.

Tan-a-ram Punthip P   Cardoso Tamara T   Daveran-Mingot Marie-Line ML   Kanchanatawee Sunthorn S   Loubière Pascal P   Girbal Laurence L   Cocaign-Bousquet Muriel M  

Applied and environmental microbiology 20101203 3


The intrasubspecies diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in an ultrafiltration cheese model. The six strains were isolated from various sources, but all exhibited a dairy phenotype (growth in ultrafiltration cheese model and high acidification rate). The six strains exhibited similar behaviors in terms of growth during cheese ripening, while different acidification capabilities we  ...[more]

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