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Omics score: 31
Gis2 studies
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ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: 
Andre Gerber
PROVIDER: E-GEOD-24005 | ArrayExpress| 2014-05-01
SECONDARY ACCESSION(S): GSE24005PRJNA130355
REPOSITORIES: GEO, ArrayExpress
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2014-05-01 | E-GEOD-15297 | ArrayExpress
Project description:With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic and WT NOD mice at different ages. At 4 and 6 weeks of age, we found up-regulation of a number of genes that are known to be induced by IFN-alpha. IFN-alpha levels and IFN-alpha-producing plasmacytoid dendritic cells were increased in the PLNs of 3- to 4-week-old NOD mice. Moreover, blockade of IFN-alpha receptor 1 in NOD mice by a neutralizing antibody at 2-3 weeks of age significantly delayed the onset and decreased the incidence of type 1 diabetes, increased the relative number of immature dendritic cells in the PLNs, and enhanced the ability of spleen CD4(+) T cells to produce IL-4 and IL-10. These findings demonstrate that IFN-alpha in the PLNs is an essential initiator in the pathogenesis of type 1 diabetes in NOD mice. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
2014-05-01 | E-GEOD-15213 | ArrayExpress
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2014-05-02 | E-GEOD-11152 | ArrayExpress
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2014-05-02 | E-GEOD-9046 | ArrayExpress
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2014-05-02 | E-GEOD-9047 | ArrayExpress
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