Genomics

Dataset Information

299

Genome-wide H3K9 acetylation state before and after VPA treatment


ABSTRACT: Genome-wide maps of the H3K9 acetylation state in embryonic stem cells (ESCs) before and after treatment with low levels of the histone deacetylase (HDAC) inhibitor valproic acid (VPA). ChIP-seq for 3 samples: untreated E14 cells, cells treated with VPA for 4 hrs and cells treated with VPA for 16 hrs. Unprecipitated DNA was used as the input control (Input).

ORGANISM(S): Mus musculus  

SUBMITTER: Eran Meshorer   Hadas Hezroni 

PROVIDER: E-GEOD-24211 | ArrayExpress | 2011-09-17

SECONDARY ACCESSION(S): SRP005133GSE24211PRJNA133743

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

H3K9 histone acetylation predicts pluripotency and reprogramming capacity of ES cells.

Hezroni Hadas H   Tzchori Itai I   Davidi Anna A   Mattout Anna A   Biran Alva A   Nissim-Rafinia Malka M   Westphal Heiner H   Meshorer Eran E  

Nucleus (Austin, Tex.) 20110701 4


The pluripotent genome is characterized by unique epigenetic features and a decondensed chromatin conformation. However, the relationship between epigenetic regulation and pluripotency is not altogether clear. Here, using an enhanced MEF/ESC fusion protocol, we compared the reprogramming potency and histone modifications of different embryonic stem cell (ESC) lines (R1, J1, E14, C57BL/6) and found that E14 ESCs are significantly less potent, with significantly reduced H3K9ac levels. Treatment of  ...[more]

Publication: 1/2

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