Transcriptomics

Dataset Information

2

Expression analysis of mouse embryo fibrobalsts lacking Tgif1


ABSTRACT: Tgif1 is a transcriptional corepressor that limits TGFβ responsive gene expression. TGFβ signaling has antiproliferative effects in several cell types, generally resulting in a G1 arrest. Mouse embryo fibroblasts (MEFs) are primary cells with limited life-span, that senesce after several passages in culture. We compared expression profiles of primary MEFs lacking Tgif1 with wild types at passage three, and with wild type MEFs at passage 5, to identify changes in gene expression due to loss of Tgif1 and due to increasing passage number. Primary MEFs were passaged on a 3T3 protocol and were harvested at passage 3 or 5, for RNA isolation and hybridization to Affymetrix microarrays.

ORGANISM(S): Mus musculus  

SUBMITTER: David Wotton   Brad J Zerlanko 

PROVIDER: E-GEOD-24225 | ArrayExpress | 2012-06-20

SECONDARY ACCESSION(S): GSE24225PRJNA132919

REPOSITORIES: GEO, ArrayExpress

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Publications

Premature senescence and increased TGFβ signaling in the absence of Tgif1.

Zerlanko Brad J BJ   Bartholin Laurent L   Melhuish Tiffany A TA   Wotton David D  

PloS one 20120413 4


Transforming growth factor β (TGFβ) signaling regulates cell cycle progression in several cell types, primarily by inducing a G1 cell cycle arrest. Tgif1 is a transcriptional corepressor that limits TGFβ responsive gene expression. Here we demonstrate that primary mouse embryo fibroblasts (MEFs) lacking Tgif1 proliferate slowly, accumulate increased levels of DNA damage, and senesce prematurely. We also provide evidence that the effects of loss of Tgif1 on proliferation and senescence are not li  ...[more]

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