Transcriptomics

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31

Expression data from differentiating ES cells expressing miR-200c and miR-141


ABSTRACT: ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline. A2.miR200c ES cells, which express miR-200c/miR-141 upon addition of doxycycline, were differentiated as embryoid bodies in six petri dishes. Half of the samples were treated with doxycycline on days 2 and 4 of differentiation, and RNA was collected from all samples on days 3, 4, and 5.

ORGANISM(S): Mus musculus  

SUBMITTER: Theresa L Murphy  Ellen M Langer   Robert C Lindsley   Kenneth M Murphy   Jennifer Gill   Mi Cai   Jennifer G Gill   Michael Kyba    

PROVIDER: E-GEOD-24289 | ArrayExpress| 2011-10-12

SECONDARY ACCESSION(S): GSE24289PRJNA129997

REPOSITORIES: GEO, ArrayExpress

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