Transcriptomics

Dataset Information

30

Expression data from differentiating ES cells expressing Snail during Wnt inhibition


ABSTRACT: ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of ES cell differentiation at early timepoints after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Total RNA was harvested from control (no doxycycline) and Snail-induced (doxycycline at day 2) cultures at 6, 12, and 24 hours after doxycycline addition (corresponding to days 2.25, 2.5, and 3 of differentiation).

ORGANISM(S): Mus musculus  

SUBMITTER: Theresa L Murphy  Ellen M Langer   Robert C Lindsley   Kenneth M Murphy   Jennifer Gill   Mi Cai   Michael Kyba   Jennifer G Gill    

PROVIDER: E-GEOD-24291 | ArrayExpress| 2011-10-12

SECONDARY ACCESSION(S): GSE24291PRJNA130001

REPOSITORIES: GEO, ArrayExpress

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