Genomics

Dataset Information

88

Seq-WA30534819_H3K4me3_N2_MXemb


ABSTRACT: modENCODE_submission_3169 We analyzed H3 lysine 4 trimethylation levels in the C. elegans genome using chromatin immunoprecipitation followed by Illumina sequencing. We used wild type (N2) mixed developmental stage embryos and performed the experiment in two biological replicates. This series consists of two biological replicates of H3K4me3 ChIP-seq experiments and their input sequences. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Kohta Ikegami  Jason Lieb   Everett Zhou   Sevinc Ercan    

PROVIDER: E-GEOD-24318 | ArrayExpress| 2013-04-03

SECONDARY ACCESSION(S): SRP003622GSE24318

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Caenorhabditis elegans chromosome arms are anchored to the nuclear membrane via discontinuous association with LEM-2.

Ikegami Kohta K   Egelhofer Thea A TA   Strome Susan S   Lieb Jason D JD  

Genome biology 20101223 12


Although Caenorhabditis elegans was the first multicellular organism with a completely sequenced genome, how this genome is arranged within the nucleus is not known.We determined the genomic regions associated with the nuclear transmembrane protein LEM-2 in mixed-stage C. elegans embryos via chromatin immunoprecipitation. Large regions of several megabases on the arms of each autosome were associated with LEM-2. The center of each autosome was mostly free of such interactions, suggesting that th  ...[more]

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