Transcriptomics,Multiomics

Dataset Information

2

Identification of differentially expressed genes in Sfmbt1-knockdown C2C12 myoblasts


ABSTRACT: Gene expression profiling was performed to identify Sfmbt1-dependent regulation in myogenic programs. To establish the magnitude of the Sfmbt1 effect on muscle cells, we have compared gene expression profiles of C2C12 cells transduced with lentiviruses expressing scramble shRNA control or shSfmbt1. Our analysis suggested that Sfmbt1 critically confers transcriptional silencing of muscle genes in myogenic progenitor cells. Two biological replicates for each sample group: C2C12 cells stably transduced with pLKO.1 expressing scramble shRNA control and C2C12 cells transduced with pLKO.1 shSFMBT1. Samples were independently cultured and RNAs were then harvested for microarray analysis.

OTHER RELATED OMICS DATASETS IN: PRJNA177954

ORGANISM(S): Mus musculus  

SUBMITTER: Jian-Liang Li   Jian L Li  Lizi Wu 

PROVIDER: E-GEOD-24346 | ArrayExpress | 2013-01-28

SECONDARY ACCESSION(S): GSE24346PRJNA177954

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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Action DRS
E-GEOD-24346.idf.txt Idf
E-GEOD-24346.processed.1.zip Processed
E-GEOD-24346.raw.1.zip Raw
E-GEOD-24346.sdrf.txt Txt
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Publications

Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program.

Lin Shuibin S   Shen Huangxuan H   Li Jian-Liang JL   Tang Shaojun S   Gu Yumei Y   Chen Zirong Z   Hu Chengbin C   Rice Judd C JC   Lu Jianrong J   Wu Lizi L  

The Journal of biological chemistry 20130124 9


SFMBT1 belongs to the malignant brain tumor domain-containing chromatin reader family that recognizes repressive histone marks and represses transcription. The biological functions and molecular basis underlying SFMBT1-mediated transcriptional repression are poorly elucidated. Here, our proteomic analysis revealed that SFMBT1 is associated with multiple transcriptional corepressor complexes, including CtBP/LSD1/HDAC complexes, polycomb repressive complexes, and malignant brain tumor family prote  ...[more]

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