Transcriptomics

Dataset Information

39

Mesenchymal Stem Cells Reduce Inflammation while Enhancing Bacterial Clearance and Improving Survival in Sepsis


ABSTRACT: Expression data from Total RNA extracted from murine spleen. Sepsis was induced in C57Bl/6J mice by cecal ligation and puncture (CLP), followed 6 hours later by an intravenous injection of Mesenchymal Stem Cell (MSC) or saline. Twenty-eight hours after CLP, plasma, bronchoalveolar lavage (BAL) fluid and tissues were collected for analyses. Total RNA was extracted using Trizol (as per manufactures' instruction) followed by clean-up procedure using Qiagen RNA easy Prep (as per manufactures instructions) In the following study we hypothesized that mesenchymal stem cells (MSCs), which have documented immunomodulatory properties, would reduce sepsis-associated inflammation and organ injury in a clinically relevant model of sepsis. To identify the molecular changes associated with decreased inflammation in CLP-injured mice treated with MSCs, we analyzed the gene expression profiles from spleens collected at 28 hours from 4 animals per group: sham/saline, CLP/saline, and CLP/MSCs.

ORGANISM(S): Mus musculus  

SUBMITTER: Patrick F Lai  Duncan J Stewart   Jack J Haitsma   Yupu Deng   Arthur S Slutsky   W C Liles   Claudia Chimisso dos Santos   Claudia C Dos Santos   Shirley H Mei    

PROVIDER: E-GEOD-24357 | ArrayExpress | 2012-06-26

SECONDARY ACCESSION(S): GSE24357PRJNA132813

REPOSITORIES: GEO, ArrayExpress

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Publications

Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis.

Mei Shirley H J SH   Haitsma Jack J JJ   Dos Santos Claudia C CC   Deng Yupu Y   Lai Patrick F H PF   Slutsky Arthur S AS   Liles W Conrad WC   Stewart Duncan J DJ  

American journal of respiratory and critical care medicine 20100617 8


RATIONALE: Sepsis refers to the clinical syndrome of severe systemic inflammation precipitated by infection. Despite appropriate antimicrobial therapy, sepsis-related morbidity and mortality remain intractable problems in critically ill patients. Moreover, there is no specific treatment strategy for the syndrome of sepsis-induced multiple organ dysfunction. OBJECTIVES: We hypothesized that mesenchymal stem cells (MSCs), which have been shown to have immunomodulatory properties, would reduce seps  ...[more]

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