Transcriptomics

Dataset Information

3

Mesenchymal Stem Cells Reduce Inflammation while Enhancing Bacterial Clearance and Improving Survival in Sepsis


ABSTRACT: Expression data from Total RNA extracted from murine spleen. Sepsis was induced in C57Bl/6J mice by cecal ligation and puncture (CLP), followed 6 hours later by an intravenous injection of Mesenchymal Stem Cell (MSC) or saline. Twenty-eight hours after CLP, plasma, bronchoalveolar lavage (BAL) fluid and tissues were collected for analyses. Total RNA was extracted using Trizol (as per manufactures' instruction) followed by clean-up procedure using Qiagen RNA easy Prep (as per manufactures instructions) In the following study we hypothesized that mesenchymal stem cells (MSCs), which have documented immunomodulatory properties, would reduce sepsis-associated inflammation and organ injury in a clinically relevant model of sepsis. To identify the molecular changes associated with decreased inflammation in CLP-injured mice treated with MSCs, we analyzed the gene expression profiles from spleens collected at 28 hours from 4 animals per group: sham/saline, CLP/saline, and CLP/MSCs.

ORGANISM(S): Mus musculus  

SUBMITTER: Patrick F Lai   Duncan J Stewart  Jack J Haitsma  Yupu Deng  Arthur S Slutsky  W C Liles  Claudia Chimisso dos Santos  Claudia C Dos Santos  Shirley H Mei 

PROVIDER: E-GEOD-24357 | ArrayExpress | 2010-11-22

SECONDARY ACCESSION(S): GSE24357PRJNA132813

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis.

Mei Shirley H J SH   Haitsma Jack J JJ   Dos Santos Claudia C CC   Deng Yupu Y   Lai Patrick F H PF   Slutsky Arthur S AS   Liles W Conrad WC   Stewart Duncan J DJ  

American journal of respiratory and critical care medicine 20100617 8


RATIONALE: Sepsis refers to the clinical syndrome of severe systemic inflammation precipitated by infection. Despite appropriate antimicrobial therapy, sepsis-related morbidity and mortality remain intractable problems in critically ill patients. Moreover, there is no specific treatment strategy for the syndrome of sepsis-induced multiple organ dysfunction. OBJECTIVES: We hypothesized that mesenchymal stem cells (MSCs), which have been shown to have immunomodulatory properties, would reduce seps  ...[more]

Similar Datasets

2012-12-31 | E-GEOD-40180 | ArrayExpress
2010-11-22 | GSE24357 | GEO
2014-02-21 | E-GEOD-55238 | ArrayExpress
2014-12-20 | E-GEOD-34790 | ArrayExpress
2011-10-14 | E-MEXP-2944 | ArrayExpress
2007-12-07 | E-GEOD-4479 | ArrayExpress
2015-05-15 | E-GEOD-47094 | ArrayExpress
2010-06-08 | E-GEOD-22009 | ArrayExpress
2007-02-15 | GSE5811 | GEO
2015-12-07 | E-GEOD-68968 | ArrayExpress