Transcriptomics

Dataset Information

2

Transcriptome response to different carbon sources in Acetobacter aceti


ABSTRACT: Acetic acid bacteria are obligately aerobic alphaproteobacteria that have a unique ability to incompletely oxidize various alcohols and sugars to organic acids. The ability of these bacteria to incompletely oxidize ethanol to acetate has been historically utilized for vinegar production. The mechanism of switching between incomplete oxidation and assimilatory oxidation and the control of energy and carbon metabolism in acetic acid bacteria are not fully understood. To understand the physiology and molecular biology of acetic acid bacteria better, we determined the draft genome sequence of Acetobacter aceti NBRC 14818, which is the type strain of the genus. Based on this draft genome sequence, the transcriptome profiles in A. aceti cells grown on ethanol, acetate, glucose, or mix of ethanol and glucose was determined by using NimbleGen Prokaryotic Expression array (4x72K). Acetobacter aceti NBRC14818 was cultivated in the medium containing ethanol, acetate, glucose, or mix of ethanol and glucose as carbon sources in Erlenmeyer flask with rotary shaking. Total RNA was extracted when optical density at 600 nm was 0.3-0.4. The experiment was performed in duplicate independent cultures.

ORGANISM(S): Acetobacter aceti NBRC 14818  

SUBMITTER: Hiroyuki Arai   Kenta Sakurai 

PROVIDER: E-GEOD-24361 | ArrayExpress | 2011-01-17

SECONDARY ACCESSION(S): GSE24361PRJNA132821

REPOSITORIES: GEO, ArrayExpress

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Transcriptome response to different carbon sources in Acetobacter aceti.

Sakurai Kenta K   Arai Hiroyuki H   Ishii Masaharu M   Igarashi Yasuo Y  

Microbiology (Reading, England) 20101116 Pt 3


The draft genome sequence of Acetobacter aceti NBRC 14818 was determined by whole-genome shotgun sequencing and the transcriptome profile in cells exponentially grown on ethanol, acetate or glucose was analysed by using a DNA microarray. The genes for all enzymes that constitute the complete tricarboxylic acid (TCA) cycle and glyoxylate pathway were identified in the genome. The TCA cycle genes showed higher expression levels in A. aceti cells grown on acetate or glucose and the glyoxylate pathw  ...[more]

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