Transcriptomics

Dataset Information

5

Secreted Factors from Staphylococcus aureus Biofilm and Planktonic Cultures Differentially Impact Human Keratinocytes in vitro


ABSTRACT: Interactions between human keratinocytes and secreted factors from Staphylococcus aureus biofilm were investigated using microarray analysis. Relative to control cells, biofilm-secreted factors upregulated cytokine and chemokine genes in keratinocytes. Genes associated with DNA damage and oxidative stress were also induced in keratinocytes treated with secreted factors from S. aureus. Here we show that secreted factors from S. aureus biofilm cultures differentially impact several aspects of wound healing processes. Human keratinocytes were grown in co-culture with mature S. aureus biofilms for 24 hours. Keratinocytes exposed to S. aureus biofilm were analyzed in quadruplicate. Control cells were also analyzed in quadruplicate. Dye-swaps were performed.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kate McInnerney   Scot E Dowd  Philip S Stewart  Philip Fleckman  Garth A James  Patrick R Secor  John E Olerud 

PROVIDER: E-GEOD-24370 | ArrayExpress | 2011-08-30

SECONDARY ACCESSION(S): GSE24370PRJNA132965

REPOSITORIES: GEO, ArrayExpress

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