Transcriptomics

Dataset Information

4

Microarray analysis of V. cholerae genes differentially expressed in 12 h rabbit ileal loop mucus


ABSTRACT: The mucus and cell-associated bacteria were isolated as a single fraction from ileal loops twelve hours post inoculation by scraping the epithelial surface of the intestine with a disposable plastic cell scraper after the loops had been cut open and gently rinsed in PBS buffer to wash away remaining luminal fluid. The mucus gel/cell associated fraction was suspended in Trizol and frozen on dry ice. Total RNA, which includes RNA from the infecting bacteria and from the host (see below), was isolated from the thawed Trizol suspension, treated with DNaseI (Applied Biosystems, Austin, TX) and cleaned by using the RNeasy kit (Qiagen, Valencia, CA). Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.

ORGANISM(S): Vibrio cholerae  

SUBMITTER: Stanford Microarray Database   Nadia Dolganov 

PROVIDER: E-GEOD-24406 | ArrayExpress | 2010-09-28

SECONDARY ACCESSION(S): GSE24406PRJNA133751

REPOSITORIES: GEO, ArrayExpress

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Publications

A bistable switch and anatomical site control Vibrio cholerae virulence gene expression in the intestine.

Nielsen Alex T AT   Dolganov Nadia A NA   Rasmussen Thomas T   Otto Glen G   Miller Michael C MC   Felt Stephen A SA   Torreilles St├ęphanie S   Schoolnik Gary K GK  

PLoS pathogens 20100916 9


A fundamental, but unanswered question in host-pathogen interactions is the timing, localization and population distribution of virulence gene expression during infection. Here, microarray and in situ single cell expression methods were used to study Vibrio cholerae growth and virulence gene expression during infection of the rabbit ligated ileal loop model of cholera. Genes encoding the toxin-coregulated pilus (TCP) and cholera toxin (CT) were powerfully expressed early in the infectious proces  ...[more]

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