Dataset Information


Murine bone marrow-derived mast cells (BMMCs) transduced with pAPM lentiviruses overexpressing miR-221 vs control overexpressing miR-221m.

ABSTRACT: Bone marrow-derived mast cells were derived from three different female C57Bl/6 mice of similar age. Cells were transduced with pAPM lentiviruses either overexpressing microRNA-221 or a non functional microRNA-221 mutant, termed miR-221m, where four point-mutations were introduced in the seed sequence of miR-221. The samples overexpressing miR-221m were used as references for the corresponding miR-221 overexpressing BMMCs from the same mouse (mouse matched reference). The experiment was performed in order to gain more insights about the molecular mechanisms underlying miR-221 effects in mast cells. Two-condition experiment, BMMC overexpressing miR-221 vs BMMC overexpressing miR-221m. Biological replicates: 3 replicates overexpressing miR-221, 3 control replicates overexpressing miR-221m.

ORGANISM(S): Mus musculus  

SUBMITTER: Silvia Monticelli   Ramon J Mayoral 

PROVIDER: E-GEOD-24462 | ArrayExpress | 2011-11-23



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MiR-221 influences effector functions and actin cytoskeleton in mast cells.

Mayoral Ramon J RJ   Deho Lorenzo L   Rusca Nicole N   Bartonicek Nenad N   Saini Harpreet Kaur HK   Enright Anton J AJ   Monticelli Silvia S  

PloS one 20111012 10

Mast cells have essential effector and immunoregulatory functions in IgE-associated allergic disorders and certain innate and adaptive immune responses, but the role of miRNAs in regulating mast cell functions is almost completely unexplored. To examine the role of the activation-induced miRNA miR-221 in mouse mast cells, we developed robust lentiviral systems for miRNA overexpression and depletion. While miR-221 favored mast cell adhesion and migration towards SCF or antigen in trans-well migra  ...[more]

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