Dataset Information


Human Peripheral blood monocytes untreated or treated with CXCL12

ABSTRACT: Total mRNA expression of CXCL12-treated monocytes was compared with control untreated monocytes Human Peripheral blood monocytes from three different healthy donors were isolated by anti-CD14-labeled magnetic microbeads. CD14+ monocytes were cultured in teflon dishes for 1h in RPMI 10% FCS and then, were treated or not with CXCL12 for 6h. Total RNA from each condition was extracted and purified using the RNasey kit (Qiagen). Labelled RNA was used as hybridization probes on human Codelink Whole genome Bioarray. All experimental procedures were performed following manufacturer instructions. Microarrays were scanned with a GenePix 4000B (Axon Instruments) scanner. Scanned images and raw data were processed using the Codelink Expression Software.

ORGANISM(S): Homo sapiens  

SUBMITTER: Lorena Sanchez-Martin   Lorena Sánchez-Martín  Paloma Sanchez-Mateos 

PROVIDER: E-GEOD-24496 | ArrayExpress | 2011-01-13



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The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression.

Sánchez-Martín Lorena L   Estecha Ana A   Samaniego Rafael R   Sánchez-Ramón Silvia S   Vega Miguel Ángel MÁ   Sánchez-Mateos Paloma P  

Blood 20101007 1

Monocytes are versatile cells that can express different functional programs in response to microenvironmental signals. We show that primary blood monocytes secrete the CXCL12 chemokine, and express the CXCR4 and CXCR7 receptors, leading to an autocrine/paracrine loop that contribute to shape monocyte differentiation to a distinct type of macrophages, with an enhanced expression of CD4, CD14, and CD163, or dendritic cells, with a reduced functional ability to stimulate antigen-specific T-lymphoc  ...[more]

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