Transcriptomics

Dataset Information

33

Identification of genes induced on nitrate, role of OxyR


ABSTRACT: The study aimed to identify role of OxyR during growth on different electron acceptors when E. coli are growing anaerobically. Wt and OxyR null cells were grown on nitrate and fumarate medium anaerobically. Each condition was done in duplicate. RNA was extracted using Qiagen RNeasy kit after stabilization of RNA with RNA protect bacteria reagent. Hybridization and further processing was done based on Affymetrix protocols on E. coli Genome 2.0 arrays.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655  

SUBMITTER: Divya Seth   

PROVIDER: E-GEOD-24524 | ArrayExpress| 2012-06-26

SECONDARY ACCESSION(S): GSE24524PRJNA132657

REPOSITORIES: GEO, ArrayExpress

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Publications

Endogenous protein S-Nitrosylation in E. coli: regulation by OxyR.

Seth Divya D   Hausladen Alfred A   Wang Ya-Juan YJ   Stamler Jonathan S JS  

Science (New York, N.Y.) 20120401 6080


Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in Escherichia coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, previously thought to operate only under aerobic conditions. Deletion of OxyR resulted in large increases in protein S-nitrosylation, and S  ...[more]

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