Transcriptomics

Dataset Information

0

Transcriptional failures arrest development after human nuclear transfer


ABSTRACT: Reprogramming occurs after nuclear transfer into zygotes whose genomes have been removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase. Our results suggest that there is a previously unappreciated barrier to successful human nuclear transfer, and that future studies should focus on the requirements for somatic genome activation. 1-3 embryos were used for analysis. RNA amplification was done using two or three rounds of T7-mediated RNA amplification using the Illumina Total Prep RNA Amplification kit. Somatic cells 1-000 and 1-011 required only one round of RNA amplification because starting amounts of RNA were 100-500ng, while embryonic samples were amplified from single cells or embryos.

ORGANISM(S): Homo sapiens  

SUBMITTER: Dieter Egli  Kevin Eggan    

PROVIDER: E-GEOD-24631 | ArrayExpress | 2011-10-03

SECONDARY ACCESSION(S): GSE24631PRJNA132461

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2011-10-04 | GSE24631 | GEO
2010-05-27 | E-GEOD-21609 | ArrayExpress
2010-05-01 | GSE21609 | GEO
2011-10-03 | E-GEOD-28811 | ArrayExpress
2014-04-27 | E-GEOD-54849 | ArrayExpress
| GSE110851 | GEO
| GSE49148 | GEO
| GSE53497 | GEO
2010-02-05 | GSE17402 | GEO
2011-10-05 | E-GEOD-28024 | ArrayExpress