Transcriptomics

Dataset Information

5

Genome-wide analysis of the effect of long-term freezing of PAXgene Blood RNA tubes


ABSTRACT: Analysis of long-term freezing on the stability of transcriptome profiles in PAXgene stabilized whole blood samples. In the present study it was tested if long-term freezing of PAXgene RNA tubes (up to one year) has an influence on the transcriptome profile of peripheral whole blood samples. Results indicated that gene expression profiles of whole blood samples stabilized with PAXgene RNA tubes remain stable for at least 1 year. Total RNA was obtained from peripheral whole blood samples collected in PAXgene RNA tubes of healthy human subjects. RNA was either isolated after 24h storage at room temperature or after freezing of PAXgene RNA tubes for up to 1 year (6 weeks, 6 months and 12 months).

ORGANISM(S): Homo sapiens  

SUBMITTER: Joachim Schultze   Andrea Staratschek-Jox  Svenja Debey-Pascher 

PROVIDER: E-GEOD-24757 | ArrayExpress | 2011-07-21

SECONDARY ACCESSION(S): GSE24757PRJNA133395

REPOSITORIES: GEO, ArrayExpress

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Publications

RNA-stabilized whole blood samples but not peripheral blood mononuclear cells can be stored for prolonged time periods prior to transcriptome analysis.

Debey-Pascher Svenja S   Hofmann Andrea A   Kreusch Fatima F   Schuler Gerold G   Schuler-Thurner Beatrice B   Schultze Joachim L JL   Staratschek-Jox Andrea A  

The Journal of molecular diagnostics : JMD 20110701 4


Microarray-based transcriptome analysis of peripheral blood as surrogate tissue has become an important approach in clinical implementations. However, application of gene expression profiling in routine clinical settings requires careful consideration of the influence of sample handling and RNA isolation methods on gene expression profile outcome. We evaluated the effect of different sample preservation strategies (eg, cryopreservation of peripheral blood mononuclear cells or freezing of PAXgene  ...[more]

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