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Omics score: 29

Expression data from Saccharomyces cerevisiae strains deleted for different subunits of the THO/TREX complex

ABSTRACT: THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis at the interface of transcription-RNA export with a key role in preventing transcription-associated genome instability. We used microarrays to analyze the impact of different THO/TREX mutations on gene expression and found that THO-Sub2 deletions have a high functional impact on highly expressed, long and G+C-rich genes regardless of gene function. S. cerevisiae strains were grown in YPAD liquid culture, total RNA was isolated and hybridized on Affymetrix microarrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andrés Aguilera Antonio Marín Hélène Gaillard Cristina Tous Belén Gómez-González

PROVIDER: E-GEOD-24802 | ArrayExpress| 2012-06-26

SECONDARY ACCESSION(S): GSE24802PRJNA132295

REPOSITORIES: GEO, ArrayExpress

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Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles.

Gómez-González Belén B García-Rubio María M Bermejo Rodrigo R Gaillard Hélène H Shirahige Katsuhiko K Marín Antonio A Foiani Marco M Aguilera Andrés A

The EMBO journal 20110624 15

THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and prevents transcription-associated recombination. Whether or not it has a ubiquitous role in the genome is unknown. Chromatin immunoprecipitation (ChIP)-chip studies reveal that the Hpr1 component of THO and the Sub2 RNA-dependent ATPase have genome-wide distributions at active ORFs in yeast. In contrast to RNA polymerase II, evenly distributed from promoter to termination regions, THO and Sub2 are absent at promoters a ...[more]

PMID: 21701562

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