Dataset Information


Expression data from Saccharomyces cerevisiae strains deleted for different subunits of the THO/TREX complex

ABSTRACT: THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis at the interface of transcription-RNA export with a key role in preventing transcription-associated genome instability. We used microarrays to analyze the impact of different THO/TREX mutations on gene expression and found that THO-Sub2 deletions have a high functional impact on highly expressed, long and G+C-rich genes regardless of gene function. S. cerevisiae strains were grown in YPAD liquid culture, total RNA was isolated and hybridized on Affymetrix microarrays.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Andrés Aguilera  Antonio Marín   Hélène Gaillard   Cristina Tous   Belén Gómez-González    

PROVIDER: E-GEOD-24802 | ArrayExpress | 2012-06-26



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Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles.

Gómez-González Belén B   García-Rubio María M   Bermejo Rodrigo R   Gaillard Hélène H   Shirahige Katsuhiko K   Marín Antonio A   Foiani Marco M   Aguilera Andrés A  

The EMBO journal 20110624 15

THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and prevents transcription-associated recombination. Whether or not it has a ubiquitous role in the genome is unknown. Chromatin immunoprecipitation (ChIP)-chip studies reveal that the Hpr1 component of THO and the Sub2 RNA-dependent ATPase have genome-wide distributions at active ORFs in yeast. In contrast to RNA polymerase II, evenly distributed from promoter to termination regions, THO and Sub2 are absent at promoters a  ...[more]

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