Transcriptomics

Dataset Information

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Transcription profiling by array of Arabidopsis wild-type and dcl1-15 torpedo-staged embryos


ABSTRACT: Severe loss-of-function alleles of DCL1 are embryonic lethal. Defects in cell division were seen as early as the globular stage in the strong loss-of-function allele dcl1-15. Phenotypic work with dcl1-15 and the null allele dcl1-5 suggested that, in addition to the severe patterning defects, the mutants were maturing earlier than wild-type embryos. We performed global gene expression analysis of dcl1-15 and wild-type torpedo staged embryos to examine this maturation phenotype further. The results suggested that DCL1 is a heterochronic gene. Comparisons to a time series of embryo development (http://www.seedgenenetwork.net/arabidopsis) showed that the genes differentially expressed in dcl1-15 embryos behaved more like green-cotyledon stage embryos than torpedo embryos. Seeds of a DCL1/dcl1-15 plant were sown. For each wild-type sample, 300 torpedo stage embryos were selected from wild-type siblings. For each mutant replicate, 300 dcl1-15/dcl1-15 embryos were selected from the siliques of DCL1/dcl1-15 where the wild-type embryos were at the torpedo stage.

REANALYSED by: E-GEOD-24887

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Pablo D Jenik   Matthew R Willmann 

PROVIDER: E-GEOD-24887 | ArrayExpress | 2011-02-17

SECONDARY ACCESSION(S): GSE24887PRJNA132035

REPOSITORIES: GEO, ArrayExpress

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Publications

MicroRNAs regulate the timing of embryo maturation in Arabidopsis.

Willmann Matthew R MR   Mehalick Andrew J AJ   Packer Rachel L RL   Jenik Pablo D PD  

Plant physiology 20110217 4


The seed is a key evolutionary adaptation of land plants that facilitates dispersal and allows for germination when the environmental conditions are adequate. Mature seeds are dormant and desiccated, with accumulated storage products that are to be used by the seedling after germination. These properties are imposed on the developing embryo by a maturation program, which operates during the later part of embryogenesis. A number of "master regulators" (the "LEC genes") required for the induction  ...[more]

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