Transcriptomics

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Escherichia coli biofilm: mono-species culture vs. mixed-species culture


ABSTRACT: Transcription profile of Escherichia coli cells in mono-species pure biofilms was compared to that of E. coli cells in E. coli-Stenotrophomonas maltophilia dual-species biofilms. E. coli cells were separated from dual-species biofilms before total RNA extraction to eliminate possible cross hybridization from S. maltophilia transcripts. The separation method was developed by combining the use of reagent RNAlater and immuno-magnetic separation. Pure E. coli biofilms were processed with the same separation protocol before RNA extraction. Two condition experiments: E. coli mono-species biofilm vs E. coli in mixed-species biofilm. Two biological replicates with independently grown and harvested biofilms. Each biological replicate has two or three technical replicates of hybridization on microarray slides. Each slide has three built-in replicates for each probe.

ORGANISM(S): Escherichia coli K-12  

SUBMITTER: Dongjuan Dai   Chuanwu Xi  Lutgarde Raskin 

PROVIDER: E-GEOD-24912 | ArrayExpress | 2013-12-30

SECONDARY ACCESSION(S): GSE24912PRJNA133403

REPOSITORIES: GEO, ArrayExpress

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