Transcriptomics

Dataset Information

80

A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo


ABSTRACT: Argonaute/Piwi proteins associate with small RNAs that typically provide sequence specificity for RNP function in gene and genome regulation. Here we show that Twi12, a Tetrahymena Piwi protein essential for growth, is loaded with mature tRNA fragments. The tightly bound ~18-22 nt tRNA 3’ fragments are biochemically distinct from the tRNA halves produced transiently in response to stress. Notably, the end positions of Twi12-bound tRNA 3' fragments precisely match RNAs detected in total small RNA of mouse embryonic stem cells and human cancer cells. Our studies demonstrate unanticipated evolutionary conservation of mature tRNA processing to tRNA-fragment small RNAs. Two libraries are analyzed here: sRNAs associated with slightly overexpressed ZZ-tagged Twi12 purified under native conditions (size range 15-34nt), and those associated after formaldehyde crosslinking (15-22nt).

ORGANISM(S): Tetrahymena thermophila  

SUBMITTER: Kathleen Collins  Ravi Sachidanandam   Mary T Couvillion    

PROVIDER: E-GEOD-24916 | ArrayExpress| 2013-04-03

SECONDARY ACCESSION(S): GSE24916SRP003894PRJNA132107

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo.

Couvillion Mary T MT   Sachidanandam Ravi R   Collins Kathleen K  

Genes & development 20101124 24


Argonaute/Piwi proteins associate with small RNAs that typically provide sequence specificity for RNP function in gene and genome regulation. Here we show that Twi12, a Tetrahymena Piwi protein essential for growth, is loaded with mature tRNA fragments. The tightly bound ~18- to 22-nucleotide tRNA 3' fragments are biochemically distinct from the tRNA halves produced transiently in response to stress. Notably, the end positions of Twi12-bound tRNA 3' fragments precisely match RNAs detected in tot  ...[more]

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