Dataset Information


MiRNA expression profiling in human mammary epithelial cell (HMEC) CD24-CD44+ and non-CD24-CD44+ cell populations

ABSTRACT: To understand the role of p53 in regulating stem cell population (CD24-CD44+) and stemness-associated miRNAs, we first compared miRNA expression profiles between human mammary epithelical cells knocked-down p53 and control cells. We then cross-referenced p53-regulated miRNAs with stemness-associated miRNAs analyzed from expression profiling of sorted CD24-CD44+ and non-CD24-CD44+ cell populations. Further biological experiments were performed with the miRNAs that are altered in CD24-CD44+ stem cell populations and also regulated by p53. Total RNAs, including miRNAs, extracted from CD24-CD44+ cells (labeled in Hy3) and non-CD24-CD44+ cells (labeled in Hy5) were hybridized on Exiqon miRCURY LNA arrays according to the manufacturer's protocol.

ORGANISM(S): Homo sapiens  

SUBMITTER: C J Chang   Mien C Hung  M C Hung 

PROVIDER: E-GEOD-25036 | ArrayExpress | 2011-02-01



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p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs.

Chang Chun-Ju CJ   Chao Chi-Hong CH   Xia Weiya W   Yang Jer-Yen JY   Xiong Yan Y   Li Chia-Wei CW   Yu Wen-Hsuan WH   Rehman Sumaiyah K SK   Hsu Jennifer L JL   Lee Heng-Huan HH   Liu Mo M   Chen Chun-Te CT   Yu Dihua D   Hung Mien-Chie MC  

Nature cell biology 20110220 3

The epithelial-mesenchymal transition (EMT) has recently been linked to stem cell phenotype. However, the molecular mechanism underlying EMT and regulation of stemness remains elusive. Here, using genomic approaches, we show that tumour suppressor p53 has a role in regulating both EMT and EMT-associated stem cell properties through transcriptional activation of the microRNA miR-200c. p53 transactivates miR-200c through direct binding to the miR-200c promoter. Loss of p53 in mammary epithelial ce  ...[more]

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